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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Hepatocyte Period 1 dictates oxidative substrate selection independent of the core circadian clock

doi: 10.1016/j.celrep.2024.114865

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: siCry1 , Santa Cruz Biotechnology , Cat#sc-44835.

Techniques: Virus, Plasmid Preparation, Recombinant, Enzyme-linked Immunosorbent Assay, Colorimetric Assay, In Vivo, Sequencing, Software

Figure 4. Fasting induces Per1, Fgf21, and Pdk4 independent of the core circadian clock (A and C) Schematic of in vitro feed/starve experiment in AML12 cells treated with either adenovirus or siRNA targeting Per1, Bmal1, Clock, or Cry1. (B) Relative expression level of Per1, Fgf21, and Pdk4 in AML12 cells treated with Ad-GFP or Ad-shPer1, fed or starved; n = 5. (D) Relative expression level of Per1, Fgf21, and Pdk4 in AML12 cells treated with lipofectamine (Control), siBmal1, siClock, or siCry1, fed or starved; n = 3. (E) Schematic of ex vivo primary hepatocyte starvation experiment from Per2WT and Per2KO female mice. (F) Relative expression level of Per1, Fgf21, and Pdk4 in primary hepatocytes isolated from Per2WT or Per2KO mice, fed or starved; n = 3–4. (G) Schematic of in vivo time-course experiment in Bmal1fl/fland Bmal1cLKO (Bmal1fl/fl, Alb-Cre) mice. Mice were either fed ad libitum or fasted for 16 h, and liver tissues were harvested every 4 h in a 24-h duration; n = 3–4.

Journal: Cell reports

Article Title: Hepatocyte Period 1 dictates oxidative substrate selection independent of the core circadian clock.

doi: 10.1016/j.celrep.2024.114865

Figure Lengend Snippet: Figure 4. Fasting induces Per1, Fgf21, and Pdk4 independent of the core circadian clock (A and C) Schematic of in vitro feed/starve experiment in AML12 cells treated with either adenovirus or siRNA targeting Per1, Bmal1, Clock, or Cry1. (B) Relative expression level of Per1, Fgf21, and Pdk4 in AML12 cells treated with Ad-GFP or Ad-shPer1, fed or starved; n = 5. (D) Relative expression level of Per1, Fgf21, and Pdk4 in AML12 cells treated with lipofectamine (Control), siBmal1, siClock, or siCry1, fed or starved; n = 3. (E) Schematic of ex vivo primary hepatocyte starvation experiment from Per2WT and Per2KO female mice. (F) Relative expression level of Per1, Fgf21, and Pdk4 in primary hepatocytes isolated from Per2WT or Per2KO mice, fed or starved; n = 3–4. (G) Schematic of in vivo time-course experiment in Bmal1fl/fland Bmal1cLKO (Bmal1fl/fl, Alb-Cre) mice. Mice were either fed ad libitum or fasted for 16 h, and liver tissues were harvested every 4 h in a 24-h duration; n = 3–4.

Article Snippet: Herzog Mouse: Fgf21fl/fl Jackson Laboratory Strain#022361 RRID:IMSR_JAX:022361 Mouse: C57BL/6J Jackson Laboratory Strain#000664 RRID:IMSR_JAX:000664 Mouse: Alb-Cre Jackson Laboratory Strain#003574 RRID:IMSR_JAX:003574 Oligonucleotides siBmal1 Santa Cruz Biotechnology Cat#sc-38166 siClock Santa Cruz Biotechnology Catsc-35075 siCry1 Santa Cruz Biotechnology Cat#sc-44835 siPpara Santa Cruz Biotechnology Cat#sc-36380 siEsrrg Santa Cruz Biotechnology Cat#sc-44705 siAtf1 Santa Cruz Biotechnology Cat#sc-29755 Fgf21 antisense oligonucleotide (ASO) IONIS Pharmaceuticals Cat#ION-256,617 qRT-PCR sequences Table S3 N/A Software and algorithms ImageJ software https://imagej.nih.gov/ij. https://imagej.nih.gov/ij.

Techniques: In Vitro, Expressing, Control, Ex Vivo, Isolation, In Vivo